• How many CD data sets do you want to process?	1234567891011121314151617181920212223242526272829303132333435363738394041424344454647484950
• Which wavelength range can be used?	Wavelength ranges to be considered can be found here.
• Unit of CD value input?	mDeg mean residue CD extinction coeff. [Δε in M¯¹ cm¯¹] mean residue elipticity [Θ in grad cm² dmol¯¹]
• Which data format do you want to use?	Upload .txt file in JASCO export format (19 lines header) Upload .txt file in AVIV CDS format (14 lines header) Upload .txt file in AVIV format (19 lines header) Upload .txt file in AVIV 60DS format (25 lines header) Upload .txt file in BP format (22 lines header) Manually enter/paste: λ [BLANK or TAB] CD-Value on next page
	⇒Example file for chosen format can be found here.

• Buffer subtraction necessary?

Subtract buffer for each sample file separately.

Subtract one buffer file for all samples.

No subtraction necessary - CD data is already blanked against buffer.

• Smoothing necessary?

Yes, smooth my data with a Savitzky-Golay filter.

Yes, smooth my data with a Savitzky-Golay filter but also plot my original data.

No, do not smooth my data.

Three examples:

Wiedemann C, Bellstedt P, Görlach M (2013). CAPITO - A web server based analysis and plotting tool for circular dichroism data. Bioinformatics 29(14): 1750-1757. [PubMed]

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